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Tissue Culture

Contents:
Background information Plant cell/tissue culture is the in vitro culture of sterilised plant cells, tissues and/or organs on a nutrient medium. Unlike animal cells, many plant cells are totipotent, meaning that each cell has the capacity to regenerate the entire plant. This fact lies at the foundation of all tissue culture work. Micropropagation is the regeneration of whole plants from small pieces of plant material. These small pieces (known as ‘explants’) are grown on sterile media and the plants produced can be potted up in soil Continue to download »

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The Role of Tissue Culture in Agricultural Diversification

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Agricultural diversification to meet our future needs call for the adoption of new technologies in agriculture. Utilization of the best cultural practices, fertilization, pest control measures will not give the necessary results without the use of best planting material. Tissue culture is now a significant horticultural propagation method which has revolutionized the horticultural industry. Use of this technique should be considered for mass propagation Continue to download »

Tissue Culture for Elimination of Lily Viruses Depending on Explant Type

Contents:
The aim of the present work was to assess the potential of lily virus elimination by tissue culture in vitro. The medium used for explants was based on Murashige and Skoog medium. Plantlets regenerated from explants were examined by electron microscopy. Plantlets from basal parts of the petals and of basal pieces of leaves used as explants resulted in the best  virus elimination. In conclusion, tissue culture is applicable for virus Continue to download »

Crop Improvement through Tissue Culture

Contents:
Plant tissue culture comprises  a set of in vitro techniques, methods and strategies that are part of the group of technologies called plant biotechnology. Tissue culture has been exploited to create genetic variability from which crop plants can be improved, to improve the state of health of the planted material and to increase the number of  desirable germplasms available to the plant breeder. Tissue-culture protocols are available for Continue to download »

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Horticultural Uses for Plant Tissue Culture

Contents:
Micropropagation Tissue culture is a term that is used to refer to the growth of plants or plant parts in sterile culture. Micropropagation is a method of propagating plants that uses very small parts of plants that are grown in sterile culture. Micropropagation is only one of a number of uses for plant tissue culture that are used in horticulture. 1. Clonal mass propagation. The important point here is that extremely large numbers of plants can be Continue to download »

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