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Micropropagation Tissue culture is a term that is used to refer to the growth of plants or plant parts in sterile culture. Micropropagation is a method of propagating plants that uses very small parts of plants that are grown in sterile culture. Micropropagation is only one of a number of uses for plant tissue culture that are used in horticulture. 1. Clonal mass propagation. The important point here is that extremely large numbers of plants can be Continue to download »

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Adventitious—Developing from unusual points of origin, such as shoot or root tissues, from callus or embryos, from sources other than zygotes. Agar—a polysaccharide powder derived from algae used to gel a medium. Agar is generally used at a concentration of 6-12 g/liter. Aseptic—Free of microorganisms. Aseptic Technique—Procedures used to prevent the introduction of fungi, bacteria, viruses, mycoplasma or other microorganisms into cultures. Autoclave—A machine capable of sterilizing Continue to download »

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Plant tissue culture comprises  a set of in vitro techniques, methods and strategies that are part of the group of technologies called plant biotechnology. Tissue culture has been exploited to create genetic variability from which crop plants can be improved, to improve the state of health of the planted material and to increase the number of  desirable germplasms available to the plant breeder. Tissue-culture protocols are available for Continue to download »

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Abstract. Floret explant of a local accession of Lilium speciosum Thunb. var. gloriosoides Baker produced subculturable totipotent calli on a Murashige and Skoog basal medium with a supplement of 3 mg/l 2,4-dichlorophe- noxyacetic acid and 0.25 mg/l benzyladenine. The calli were able to form bulblets, which subsequently developed into plantlets on the MS basal medium supplemented with 0.1 mg/l naphthalene acetic acid, 1 g/l active Continue to download »

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Agricultural diversification to meet our future needs call for the adoption of new technologies in agriculture. Utilization of the best cultural practices, fertilization, pest control measures will not give the necessary results without the use of best planting material. Tissue culture is now a significant horticultural propagation method which has revolutionized the horticultural industry. Use of this technique should be considered for mass propagation Continue to download »