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Abstract. Floret explant of a local accession of Lilium speciosum Thunb. var. gloriosoides Baker produced subculturable totipotent calli on a Murashige and Skoog basal medium with a supplement of 3 mg/l 2,4-dichlorophe- noxyacetic acid and 0.25 mg/l benzyladenine. The calli were able to form bulblets, which subsequently developed into plantlets on the MS basal medium supplemented with 0.1 mg/l naphthalene acetic acid, 1 g/l active Continue to download »

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To establish an efficient regeneration system for at the stem base. These tissues were subcultured on a Anthurium andreanum cv Rubrun, seeds from plant medium supplemented with 8.9 µ M BA and 2.7 µ M spadixes were germinated on a medium supplemented NAA. After 6 weeks of culture, about 43.8 plantlets per with 2.2 µ M BA. After 2 weeks, 74% of the seeds square cm of callus were obtained. Anatomical studies germinated and four weeks later, micro-cuttings from showed the organogenic nature Continue to download »

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Background information Plant cell/tissue culture is the in vitro culture of sterilised plant cells, tissues and/or organs on a nutrient medium. Unlike animal cells, many plant cells are totipotent, meaning that each cell has the capacity to regenerate the entire plant. This fact lies at the foundation of all tissue culture work. Micropropagation is the regeneration of whole plants from small pieces of plant material. These small pieces (known as ‘explants’) are grown on sterile media and the plants produced can be potted up in soil Continue to download »

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Adventitious—Developing from unusual points of origin, such as shoot or root tissues, from callus or embryos, from sources other than zygotes. Agar—a polysaccharide powder derived from algae used to gel a medium. Agar is generally used at a concentration of 6-12 g/liter. Aseptic—Free of microorganisms. Aseptic Technique—Procedures used to prevent the introduction of fungi, bacteria, viruses, mycoplasma or other microorganisms into cultures. Autoclave—A machine capable of sterilizing Continue to download »

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